De novo transcriptome assembly of the grain-eating pest, Plodia interpunctella and its natural viral pathogen Plodia interpunctella granulosis virus

The host species used in our experiment was Plodia interpunctella, the Indian Meal Moth. All individuals were taken from a large stock that has been maintained at the University of Sheffield for approximately 8 years. These transcriptomes were built from RNA-seq reads that originated from two experiments. The first compared larvae that had been exposed to virus with those that we exposed to a control solution (N=6 samples). The second compared larvae that had received two doses of virus compared to a single dose. In all cases, fourth instar larvae were harvested, and each sample consisted of 20 pooled individuals.

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